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KMID : 1001920170600040391
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Journal of Korean Neurosurgical Society
2017 Volume.60 No. 4 p.391 ~ p.396
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Ginkgolide B Modulates BDNF Expression in Acute Ischemic Stroke
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Wei Hu
Sun Tao
Tian Yang Hua
Wang Kai
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Abstract
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Objective: To investigate the neuroprotective effects of Ginkgolide B (GB) against ischemic stroke-induced injury in vivo and in vitro, and further explore the possible mechanisms concerned.
Methods: Transient middle cerebral artery occlusion (tMCAO) mice and oxygen-glucose deprivation/reoxygenation (OGD/R)-treated N2a cells were used to explore the neuroprotective effects of GB. The expression of brain-derived neurotrophic factor (BDNF) was detected via Western blot and qRT-PCR.
Results: GB treatment (4 mg/kg, i. p., bid) significantly reduced neurological deficits, water content, and cerebral infarct volume in tMCAO mice. GB also significantly increased Bcl-2/Bax ratio, reduced the expression of caspase-3, and protected against OGD/R-induced neuronal apoptosis. Meanwhile, GB caused the up-regulation of BDNF protein in vivo and in vitro.
Conclusion: Our data suggest that GB might protect the brain against ischemic insult partly via modulating BDNF expression.
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KEYWORD
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Stroke , Ginkgolide B , Brain-derived neurotrophic factor , Apoptosis
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